目的 聚羟基脂肪酸酯是替代传统包装材料的一种有前景的生物聚酯,为了降低其成本,研究了盐单胞菌利用橡胶木水解液和戊酸共基质合成3-羟基丁酸-3-羟基戊酸共聚酯（PHBV）的优化工艺。方法 制备橡胶木水解液,以橡胶木水解液和戊酸作为盐单胞菌Halomonas venusta DSM4743的碳源合成PHBV。定期测定培养基内总还原糖和戊酸残留率和盐单胞菌胞内PHBV、3-羟基丁酸酯（3HB）和3-羟基戊酸酯（3HV）含量,探讨氮源种类、NaCl浓度和pH值对PHBV合成工艺的影响。结果 以氯化铵为氮源,氯化钠浓度为60 g/L、pH值为7时,盐单胞菌合成PHBV的产量最大,在120 h时的PHBV产量达到2.01 g/L,其中HV单体占比为21.45%,PHBV为菌体干重的73.05%。结论 在此优化条件下,降低了合成PHBV的生产成本,提高了PHBV的产量,具有很好的应用价值。
Abstract
Polyhydroxyalkanoate is a promising alternative to traditional packaging materials as a biopolymer. In order to reduce its cost, the work aims to study the optimization process of synthesizing 3-hydroxybutanoic acid-3-hydroxyvaleric acid copolyester (PHBV) by halophilic bacteria with rubber wood hydrolysate and valeric acid as a co-matrix. The rubber wood hydrolysate was prepared and the PHBV was synthesized with rubber wood hydrolysate and valeric acid as carbon sources for Halomonas venusta DSM4743. The residual rates of total reducing sugars and valeric acid in the culture medium, as well as the intracellular contents of PHBV, 3-hydroxybutyrate (3HB), and 3-hydroxyvalerate (3HV) in halophilic bacteria were measured regularly, to explore the effects of nitrogen source types, NaCl concentration, and pH value on the synthesis process of PHBV. When ammonium chloride was used as the nitrogen source, sodium chloride concentration was 60 g/L, and pH value was 7, the yield of PHBV synthesized by halophilic bacteria was the highest. At 120 hours, the PHBV yield reached 2.01 g/L, with HV monomer accounting for 21.45% and PHBV being 73.05% of the dry weight of the bacterial cells. Under these optimized conditions, the production cost of synthesizing PHBV has been reduced and the yield of PHBV has been increased, presenting good application value.
关键词
3-羟基丁酸-3-羟基戊酸共聚酯（PHBV） /
盐单胞菌 /
共发酵 /
橡胶木水解液 /
戊酸
Key words
poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) /
halophilic bacteria /
co-fermentation /
rubber wood hydrolysate /
valeric acid
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基金
青海理工大学（筹）“昆仑英才”人才引进科研项目（2023-QLGKLYCZX-020）
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