目的 针对嗜盐菌对挥发性脂肪酸（Volatile fatty acid,VFAs）的耐受能力有限（通常<5 g/L）,限制了聚羟基脂肪酸酯（Polyhydroxyalkanoate,PHA）积累效率,开发一种具有高VFAs耐受的菌株,并深入解析嗜盐菌对VFAs的代谢特性,以提升PHA的生产效率。方法 首先,通过设置不同VFAs浓度梯度,探讨该菌株对不同VFAs的耐受度;其次,通过比较不同VFAs作为碳源时的PHA产量、PHA得率以及PHA生产效率,明确了该菌株的最适碳源种类;最后,通过分析发酵周期中碳源利用率、消耗速率等参数,揭示该菌株对VFAs依赖性的代谢规律。结果 Halomonas venusta对VFAs的耐受性依次为丁酸（50 g/L）>乙酸/戊酸（15 g/L）>丙酸。30 g/L丁酸为最佳PHB合成碳源（PHB产量为1.81 g/L,PHB质量分数为为73.98%）,15 g/L戊酸最适于PHBV的合成（PHBV产量为1.01 g/L,PHBV质量分数为59.41%）。代谢分析显示双峰消耗特征,且Yx/s和Yp/s与碳源浓度呈负相关。结论 较于其他嗜盐菌株,Halomonas venusta对VFAs整体表现出更优异的耐受能力,有效提高了PHA生产效率。该研究为优化PHA生产工艺提供了理论依据和数据支撑。
Abstract
The work aims to develop a Halomonas venusta strain with exceptional VFAs tolerance and systematically elucidate its VFAs metabolic mechanisms, to address the critical issue of limited volatile fatty acid (VFAs) tolerance (typically<5 g/L) in halophilic bacteria that restricts polyhydroxyalkanoate (PHA) accumulation efficiency, so as to improve the production efficiency of PHAs. First, by establishing concentration gradients of different VFAs, the tolerance of the strain to various VFAs was examined. Second, by comparing PHA production, PHA yield, and PHA productivity when different VFAs were used as carbon sources, the optimal carbon source type for the strain was identified. Finally, by analyzing parameters such as carbon source utilization rate and consumption rate during the fermentation cycle, the metabolic patterns of the strain's dependence on VFAs were revealed. Results demonstrated significant differential VFAs tolerance in Halomonas venusta: butyrate (50 g/L) > acetate/valerate (15 g/L) > propionate. Optimal production conditions yielded 1.81 g/L PHB (73.98 wt%) with 30 g/L butyrate and 1.01 g/L PHBV (59.41 wt%) with 15 g/L valerate. Metabolic analysis revealed characteristic biphasic consumption patterns and significant negative correlations between both biomass yield (Yx/s) and product yield (Yp/s) with carbon source concentration. In conclusion, compared with other halophilic strains, Halomonas venusta exhibits overall superior tolerance to VFAs, effectively improving PHA production efficiency. This study provides a theoretical basis and data support for optimizing the PHA production process.
关键词
聚羟基脂肪酸酯 /
Halomonas venusta /
生产效率 /
挥发性脂肪酸
Key words
polyhydroxyalkanoates /
Halomonas venusta /
production efficiency /
volatile fatty acids
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基金
海南省重点研发项目（ZDYF2025SHFZ056）;天津市自然科学基金重点项目（23JCZDJC00620）;青海理工大学“昆仑英才”人才引进科研项目资助（2023-QLGKLYCZX-020）
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