The work aims to test and analyze the performance of helical rotor pump (HRP) operating at different speeds. Three-dimensional model of HRP and mathematical model of theoretical flow rates were built. Prototype of HRP was processed and assembled. Hardware and software system used to test the performance of HRP was established. The signals were controlled and captured by Labview. The actual flow rates and flow rate fluctuation of HRP linearly increased with the increase in the rotational speeds. Flow rate fluctuation was observed and went up to 1.76 L/min from 0.43 L/min when the rotational speed increased to 8400 r/min from 2700 r/min. When the rotational speed was lower than 5800 r/min, the increase of rotational speed was basically linear with the increase of the actual flow rate. However, the actual flow rate basically no longer increased when the rotational speed exceeded 7000 r/min. The actual output flow and flow rate fluctuation of the HRP increase with the increase in the rotational speed. The increase of actual output flow improves the output pressure of the HRP; however, the increase of flow rate fluctuation decreases the stability of the HRP.