The work aims to analyze the effect of valve port flow channel structure parameter on the liquid flow field to solve the constrained multi-objective optimization problem of minimizing the maximum pressure loss, maximum liquid flow rate and maximum turbulent kinetic energy, for the purpose of improving the filling performance of isobaric filling valve. Based on the orthogonal experimental design and flow field simulation software Fluent, the flow field of filling valve port flow channel conducted the numerical simulation. Through the regression analysis, the empirical equation of maximum pressure loss, maximum liquid flow rate and maximum turbulent kinetic energy was developed with the valve port flow channel structure parameters as the independent variables. Then, the constrained multi-objective optimization model of valve port flow channel structure parameters was established. The multi-objective artificial bee colony algorithm was used to solve the optimization model. There was a conflict among the minimizing of the maximum pressure loss, maximum liquid flow rate and maximum turbulent kinetic energy and such minimizing could not be optimized at the same time. The optimal Pareto solution set of valve port flow channel structure parameters was obtained based on the multi-objective artificial bee colony algorithm. In conclusion, the multi-objective artificial bee colony algorithm can be effectively used for the optimization of the isobaric filling valve port flow channel structure parameters.