The work aims to establish the high performance liquid chromatographic method for the migration determination of 12 antioxidants in polypropylene plastic lunch boxes. 12 antioxidants in polypropylene plastic lunch box were extracted with seven food stimulants such as distilled water, 3% (mass fraction) acetic acid solution, 10% (mass fraction) ethanol solution, 20% (mass fraction) ethanol solution, 50% (mass fraction) ethanol solution, isooctane, poly 2, 6-phenylethane, determined by HPLC-DAD and quantified in the external standard method. The results showed that, within the range of 5.0~100.0 mg/L (mass concentration), 12 antioxidants had good linear relation, R2≥0.9995, the detection limit was 0.5~2.0 mg/L, the average recovery rate was 87.1%~98.7%, and the relative standard deviation was 0.8%~3.1%. The method (HPLC) used to determine the migration of 12 antioxidants in polypropylene plastic lunch boxes is simple, rapid and sensitive, and it can provide reference for the analysis on the antioxidants in food contact materials.