The work aims to put forward a scheme of automatic nail polish packaging production line at the request of some nail polish production enterprise, and develop the packaging production line for the automatic packaging production of nail polish, in order to improve the efficiency of the nail polish packaging production line, reduce the labor cost and achieve the goal that machine replaces manpower. Through the analysis on the nail polish packaging production process of some nail polish production enterprise, SolidWork software was adopted to respectively establish the 3D models for the empty bottle placing device, device used to place steel ball into empty bottle, nail polish injection device, bottle cap placing device, cap screw-in device and finished product output device, etc., and design the drawings for the parts composed of each device. Siemens S7-200 PLC was used to control the coordination operation of AC motor and cylinder of each mechanism, so as to achieve the automatic packaging production of nail polish. An automatic packaging production line of nail polish was developed, which achieved the automatic packaging production of nail polish and met the enterprise requirements. Such packaging production line of nail polish has solved the difficulty of automatic packaging of nail polish, which achieves the goal that machine replaces manpower and improves the packaging production efficiency of nail polish.