The work aims to prepare the gelatin-chitosan edible film, so as to provide the experimental data and theoretical basis for the different practical applications. Based on the features of gelatin and chitosan molecular structures and the performance structure-activity relationship of such additives as polyethylene glycol 400 (PEG-400), the gelatin-chitosan composite film was prepared by the solution casting, so as to study the effect of the gelatin-chitosan total concentration and mass ratio and the concentration of PEG-400 on the performances of the composite film. Moreover, the response surface analytical test was designed according to the combination design principle of Box-Benhnke center to discuss the interaction of all factors and search out the best technological formula of gelatin-chitosan film. The physical property of the film was greatly affected by the gelatin-chitosan mass fraction, mass ratio of gelatin and chitosan, mass fraction of PEG-400 and interactions thereof. Such excessively small or significant effects would not be favorable for the improvement of the film. When the gelatin-chitosan mass fraction was 8.5%, the mass ratio of gelatin and chitosan was 7∶3; when the mass fraction of PEG-400 was 10%, the tensile strength of the film was 19.53 MPa and the elongation at break was 39.82%. The transparency, lustrousness, surface smoothness, smell and physical property of the prepared film are well characterized.