To obtain an optimum formula and steps of a transparent fluorescent anti-counterfeiting stamp-ink and study factors affecting its physical and anti-counterfeiting indicators. A series of anti-counterfeiting stamp-inks for plastic films were prepared with self-made fluorescent powders based on rare earth. The effects of the contents of resin, fluorescent powders (EA) and 370M on the drying, blocking resistance, adhesion and fluorescence properties of imprinted samples were investigated in order to screen out an optimum formula whose properties were compared with those of a imported product (market sample). The resin molecule could increase the intensity of luminescent powders, while EA and 370M could improve the three physical properties. The best mass fraction of EA and 370M was 1.5%. The drying rate of the optimum stamp-ink was 25% faster than that of the counterpart, while its blocking resistance was 48% higher than that of the counterpart. Its adhesion strength was similar to that of the counterpart. The imprinted sample was transparent. It had high invisibility and anti-counterfeiting property very different to that of the counterpart. It showed the selection on the measure wavelength. Its anti-counterfeiting property was also superior to that of the counterpart. Its grade of light resistance was V which was I grade higher than that of the counterpart. The overall performance of the optimum formula is better than the counterpart, which might apply to the transparent plastic films outside packing materials of cigarette, alcohol and cosmetics to guard against falsification.