To analyze the basic vibration characteristics of typical ammunition system during highway transportation. Based on a typical ammunition system, the transportation vibration simulation experiment was designed to test vibration acceleration response of projectile body and key element to future analyze PSD and vibration transmissibility of ammunition system in each layer. The response of bottom ammunition and projectile body were prominent, and the vibration transmissibility had multiplex modes of apparent primary and secondary features. The peak transmissibility was 5.681 and 4.054 respectively. The results provide basic data for packing improvement and design of similar ammunition system in highway transportation.