The paper aims to blend Microfibrillated cellulose (MFC) and polylactic acid (PLA) to form membrane to improve moisture permeability, oxygen permeability and light resistance, etc. of the film to meet requirements on packaging of fruits, vegetables, etc. MFC was prepared by enzymatic hydrolysis and mechanical treatment. Coupling agent KH560 was applied to have hydrophobic modification treatment of MFC. The modified PLA (MFC-S) was mixed with PLA to prepare thin film. When the MFC-S content was 0.75%, the tensile strength of MFC-S/PLA blend film was 13.3% more than that of the pure PLA film. When the content of MFC-S was 2%, the oxygen permeability coefficient of MFC-S/PLA blend film was 1.43 times of the pure PLA film, its moisture permeability coefficient was 1.26 times of the pure PLA film, and its light transmittance was reduced by 60%. It had good light blocking effect. The packaging film has good tensile strength when the content of MFC-S is 0.75%; the film has good oxygen permeability, moisture permeability and light resistance when the content of MFC-S is 2%.