The work aims to prevent oil from contacting with oxygen during filling process and minimize the oxygen in container after sealing, so as to prolong the shelf life of oil. Based on the analytical comparison of the present situation of the oil antioxidation storage technology, the oil antioxidation protection in filling-transfer-sealing station was achieved through the antioxidative filling process design and structural optimization design. Oil antioxidation technology designed in this paper could realize the deoxygenizing/oxygen-barrier protection in filling, transferring and sealing process, to avoid the shortcomings of single station antioxidation protection in the traditional process. To ensure the smooth implementation of this new antioxidative oil filling technology, the structural design of filling valve, transferring protection mechanism and sealing mechanism was emphasized in the paper. The antioxidation protection effect of antioxidative oil filling machine designed in this paper is comprehensive and practical, and has great promotion value.