It aims to study the layout relationship of between genetic algorithm and the mobile terminal interface layout in optimal comfort a subset of the biggest characteristic. By using analytic hierarchy process (AHP) of 3D and 2D, common collaboration human-computer constraint conditions, using the genetic algorithm to the interface layout for feature selection, the influence on the interface layout in optimal comfort a subset of the biggest characteristic is found out. Through usability testing, finally it is concluded that the main points of the mobile terminal interface layout optimization design.