The work aims to study the best inclusion technology of microcapsules of eucalyptus essential oil. β-Cyclodextrin was taken as wall material for preparing microcapsules of eucalyptus essential oil. The optimal inclusion technology was determined by single factor and orthogonal test. The most appropriate conditions were: inclusion temperature 40 ℃, inclusion time 1.5 h, mass concentration of β-Cyclodextrin 8 g/mL, under which, the inclusion efficiency was 70.33% and microcapsule yield was 86.27%. The controlled release test results showed that microcapsules could greatly reduce the releasing rate of eucalyptus essential oil. The release course of microcapsules could fit well to the Avrami’s equation. The experiment has achieved its intended purpose. Microencapsulation helps improve the utilization of eucalyptus essential oil and meanwhile prolong its service life.