The work aims to research the process to prepare antibacterial film mainly composed of chitosan and its performance. The antibacterial film was produced by chitosan, polyvinyl alcohol (PVA), nano titanium dioxide (nano-TiO2) and glycerin with tape casting technique. With four factors as experimental factors, including the mass ratio of chitosan and PVA, the amount of nano titanium dioxide and glycerin dosage and the drying temperature, based on the orthogonal experiment, the performance of the film was evaluated, with antimicrobial property, water solubility and mechanical property as the indicators. When the mass ratio of chitosan and PVA was 8∶2, the mass fractions of nano- TiO2 and glycerol were respectively 0.75% and 0.5%, and the drying temperature was 70℃, the antibacterial composite film had the best comprehensive performance. The antibacterial film added with PVA and nano-TiO2 and mainly composed of chitosan has better antimicrobial property, water solubility and mechanical property, and it also has antimicrobial slow-release property.