The work aims to provide theoretical basis and guidance for the bearing design and tolerance selection, so as to effectively solve the problem regarding the selection of bearing precision tolerance. By taking 6300 deep groove ball bearing in the high-speed box pasting machine as a specific analytical example, a mathematical model of deep groove ball bearing was established, and the influences brought by micro variation of bearing parameters and their change law were analyzed. Moreover, the simulation analysis and verification concerned were conducted through software. The research showed that the fatigue life, stiffness, number of rollers and diameter of deep groove ball bearing and the curvature radii of its inner and outer rings were closely related to one another. The bearing fatigue life was sensitive to the change in the number of rollers and roller diameter, with the change of fatigue life up to 77.78%. Bearing radial stiffness was sensitive to the change in the number of bearing rollers, with the variation of 23.08%. However, bearing axial stiffness was more sensitive to the change in roller diameter and curvature radius of bearing ring, with the variations of respectively 38.29% and 15.1%. In a certain range, deep groove ball bearing with more rollers, larger diameter and smaller curvature radii of inner and outer rings should be preferably selected and designed, so as to achieve the optimal fatigue life and stiffness performance.