The work aims to obtain the transverse and longitudinal deformations on different areas of the paper of various colors in the offset printing process through experimental design, so as to provide additional data to support the high-precision printing. The properly designed cross lines were distributed on the different areas of the paper. Then experiment specimen papers were extracted for image capture, color separation, refinement, calculation and data preprocessing of cross lines to obtain relevant data. The research results could guide the practical production: longitudinal thread paper should be used in the printing process as much as possible; the layout requiring higher quality should be prioritized and put on the gripper edge in the process of typesetting; in the case of deformation compensation, the various deformations of each printing plate should be considered; meanwhile, the deformations on different areas of the papers for each printing plate were different. According to the data, the general trend of the paper deformation, the deformation distribution map on different areas of the paper and the deformation size of gripper edge, central part and tail of the sheet can be calculated out.