The work aims to solve such quality problems as peeling, cracking and filling in during the hot stamping process of acrylic substrate. Based on the new technology integrating screen printing and thermal transfer printing, the screen process and stamping process were adjusted, and the mixing proportion of gold stamping pulp and auxiliary agent was determined, the drying temperature and time as well as the temperature of stamping were determined through plenty of experiments and data analyses. The results showed that when the mass ratio of gold stamping pulp, curing agent and diluent was 10∶2.5∶1.2, the effect of hot stamping was the best and no peeling of hot-stamping acrylic foil would occur. The optimal drying temperature of gold stamping pulp was 80 ℃ and the best drying time was 45 minutes. When the temperature of drying and hot stamping was 130～150 ℃, the gold stamping had the best effects and problems like cracking or filling in of hot-stamping acrylic foil would not occur. The mass ratio of gold stamping pulp and auxiliary agent, drying temperature and time as well as stamping temperature of gold stamping pulp are the key factors to determine the hot stamping quality of acrylic substrate.