The work aims to study the spectral coloring characteristics of the halftone printing color so as to provide estimation model for the reproduction of spectrum of halftone printing color. The spectral reflection curve’s characteristics of halftone printing color of different tone values were analyzed. The characterized bands were found out according to spectral derivative of halftone printing color and then wave-length partition was carried out. Dot gain compensation and polynomial algorithm in various wave-length partitions were respectively used to estimate the spectral reflectance of halftone printer. The maximum of spectrum estimated with the halftone spectrum estimation algorithm proposed and the measured sRMS was 0.02. The average color difference between the corresponding estimated color and measured color was less than 1.5ΔE76. The accuracy of spectral estimation of halftone reproduction color can be improved with spectrum estimation model of wave-length partition. The spectral characterization of halftone colorful output device can use the estimation model herein as the forward characterization model.