To increase the throughput and improve the performance, adding shortcuts and optimizing the different amounts and locations of shortcuts layout in unidirectional circulation-type material handling system on a single loop can provide the shorter route and also improve the efficiency of the system. This paper made a mathematical model of this system, analyzed the different amounts and locations of short-cut layout and then made the simulation experiments under the condition of two basic scheduling rules. According to the analysis and experiment, under the condition of two basic scheduling rules, the first layout of shortcuts setting could decrease the interference of the system but lower the throughput. The fourth layout of shortcuts setting could maximize the throughput of the system but increase the interference. In conclusion, from the view point of decreasing the energy consumption (interference), when the only shortcut must be located in the middle of all the processing station, the interference time achieves the minimum while the throughput is the lowest. From the view point of increasing the throughput in the system, when all the shortcuts must be located in the middle of each two processing stations, the maximum throughput can be achieved while the interference time is increased.