The work aims to research the preparation of formaldehyde-free starch adhesive with sodium tripolyphosphate instead of formaldehyde. A kind of environmental friendly starch adhesive was produced by using starch, polyvinyl alcohol (PVA) and polyacrylamide (PAM) as principal raw material, sodium hypochlorite as oxidizing agent, water as solvent, and sodium tripolyphosphate as cross-linking agent. Single-factor experiment was carried out to investigate the effect of main factors, such as mass of polyacrylamide, kind of oxidizing agent, mass of oxidizing agent and mass of water on starch adhesive. The results showed that the optimal parameters were 9 g starch, 1 g polyacrylamide, 2.5 mL sodium hypochlorite as oxidizing agent, 160 mL water and pH=8～9. In conclusion, the environmental friendly adhesive has stable performance and good bonding effect. It is suitable for use in industrial process.