The work aims to determine the vibration level (PSD curve) during cart shipping and provide the difference in vibration levels during truck and cart shipping by comparing experiments, so as to provide reliable reference for a better design of transportation packaging. An instrument DER-1000 was used to record the experimental data. Firstly, a preliminary experiment was carried out to clarify the grms during the cart shipping. Secondly, two testing packages that represent light and weight package were designed. The vibration data in different road conditions and different fixed statuses and the real road transporting data by delivering the two testing packages with truck from Rizhao to Shanghai were measured in turn. By comparing the corresponding grms and PSD curve, the difference of the vibration levels between the truck and cart shipping stages was compared and analyzed. The vibration level of the cart shipping is significantly higher than that of the truck transportation. The gap of the PSD level is approximate 10 times the size of truck transportation. Thus, it is necessary to consider the vibration during the cart shipping when we design a transportation package.