The work aims to study the influence of dispersion process on water-based flexo ink dispersivity through different pre-dispersion rotating speeds, pre-dispersing time, and the ratio and milling time between zirconia balls and the printing ink. Laser partied size instrument was employed to evaluate the conditions for dispersion process and the influence of technological parameters on the dispersibility of ink through measuring 95% particle size and distribution of ink. When pre-dispersion rotating speed was 2000 r/min, pre-dispersing time 40 min, mass ratio 2︰1, and milling time 2 h, 95% particle of ink had the smallest size, the most narrow distribution and the best dispersion. In conclusion, the pre-dispersion rotating speeds, the pre-dispersing time and the ratio and milling time between zirconia balls and the printing ink have great influence on the property of the water-based flexo ink.