It aims to provide a scientific basis in determining amount of oil encapsulated in essential oil microcapsules by comparing three determination methods. Amount of the encapsulated oil was determined by weight loss method, distillation method and ultraviolet spectrophotometry. Then the three methods were compared and analyzed. The three methods all had good precision and accuracy with RSDs no more than 5% and indexed recovery at 90%-120%. The values determined by weight loss method were higher than the others. Ultraviolet spectrophotometry method was complex and had high cost, but had high detection speed. Distillation method was easy to operate and had low cost, but time-consuming was more. In conclusion, the three methods are all suitable for the amount determination of the oil encapsulated in essential oil microcapsules, but distillation method and ultraviolet spectrophotometry method have better precision and accuracy.