A combination piezoelectric nozzle of a roller biochip microarrayer based on inkjet printing technology was researched and developed, and the design of combination nozzle and the method for continuous biochip preparation using this nozzle were emphatically described. This method used the combination piezoelectric nozzle instead of the traditional single needle, continuous spotting instead of the traditional static spotting, and simultaneous spraying of multiple samples instead of spraying of single sample. A touch screen controller based on ARM9 S3C2416 microprocessor was used to control the motion of the system, finally, the accuracy of continuous preparation of biochip using the nozzle was tested by a laser tracker. The test results showed that the positioning accuracy was 8 μm and the repeatability accuracy was 5 μm， which both met the accuracy requirement. This article provides a new method to prepare biochips with simple structure, reliable method, high accuracy and high cost-effectiveness.