This work aimed to study the oxygen transmission rate of aseptic packaging bags with different thickness, materials, temperatures and humidity. The thickness of aseptic packaging bags was tested by film thickness gauge; the materials were detected by infrared spectrometer; the oxygen transmission rate was tested by OX-TRAN Model. And the influences of the thickness, material and temperature and humidity on the oxygen transmission rate were compared. The oxygen transmission rate of aseptic packaging bags decreased with the increase of the thickness. Material had significant effect on the oxygen transmission rate of aseptic packaging bags. The oxygen transmission rate increased with the increasing temperature and the gas barrier properties decreased. The thickness, materials of aseptic packaging bags and ambient temperature all had impacts on the oxygen transmission rate, while the humidity had no significant effect on the oxygen transmission rate.