This work aimed to prepare copper doped nano TiO2 antibacterial material, determine its metal dissolution rate and study its photocatalytic activity and antibacterial properties. Copper doped titanium dioxide (TiO2&Cu) nano material was prepared by hydrothermal synthesis, The catalytic kinetics of standard curve method was used to determine the copper ion dissolution rate of the material. The photocatalytic activity was determined using methylene blue as the photodegradation material. The antibacterial properties were studied under natural light and ultraviolet light irradiation using Staphylococcus aureus(ATCC25923) as the experimental bacteria. The copper ion dissolution rate reached the maximum of 72.36% on the fourth day. Under natural light and ultraviolet light irradiation, the degradation rate of methylene blue was 95.06% and 85.08%, respectively. The concentration of the material reached 10 mg/ml under light irradiation, and the bacterial inhibition rate was 94% after 90 min incubation. The TiO2&Cu material containing 0.2% Cu prepared by freeze-drying method had a good photocatalytic activity and good antibacterial properties in both the dark condition and under ultraviolet irradiation.