This work aimed to study the effect of ink-jet printing process on the conductive properties of conductive ink. Based on EPSON 1390 universal plate printer, the plastic type, print resolution, printing times, printed circuit shape were selected as the main process indicators. Single factor experiments were used to test the resistance values of the printed circuit under conditions of different factors. The molecular structure of the surface of plastic film had a direct effect on the ink conductivity, and the conductivity was improved by the plastics with higher surface tension, as it could obtain good print results. The connection precision of the ink layer was improved through higher printing resolution, thereby the conductivity was increased. The average resistance was reduced by increasing the printing times, which was beneficial to the formation of clear and complete printed circuit. The printed electronic circuit with different shapes did not have the same conductivity, and the order of electrical conductivity was: straight type > broken line > rectangular wave pattern > ring. Ink-jet printing technology had a certain effect on the conductivity of conductive ink, and the appropriate printing process would help improve the electrical conductivity of the conductive circuit.