The preparation process of an antimicrobial starch-based film containing microcapsule was introduced, and the properties of the film were investigated. In this paper, starch was used as the base material of film, and then antimicrobial microcapsules as well as auxiliary agents including gelatin, glycerin and CaCl2 were added into it to prepare the antimicrobial starch-based films containing microcapsule by casting technique. Using m(starch) ∶ m(gelatin) ∶ m(glycerin), content of microcapsule and CaCl2, reaction temperature as factors, orthogonal experiments were conducted. After the preparation, the water solubility, mechanical property and antimicrobial property were selected as indicators to test the films and evaluate the comprehensive performance of the films. When m(starch)∶m(gelatin)∶m(glycerin) was 6∶3∶1, the content of microcapsule was 20%, the content of CaCl2 was 2%, and the reaction temperature was 50 ℃, the film had relatively good comprehensive performance. The starch-based films incorporated with microcapsule showed good antimicrobial property.