This study was aimed to improve the antielectrostatic properties of the cellulose membrane, and expand its packaging application. Nano-Fe3O4/cellulose composite packaging membranes were prepared by using in-situ co-precipitation method, with the wooden cellulose as raw material. The mechanical properties, water resistance and anti-electrostatic properties of the Fe3O4/cellulose composite packaging membranes were investigated. The results showed that with increasing concentration of ferric ion, the mechanical properties and the water resistance of Fe3O4/cellulose composite membranes increased from 10.57 MPa and 0.27 to 14.78 MPa and 0.38, respectively, while the surface resistivity of the composite membranes gradually decreased. Compared to the cellulose membrane, the composite membranes had better anti-electrostatic properties, and the anti-electrostatic properties were more stable in low humidity environment.