This work was aimed to design the artistic dot with micro-structure anti-counterfeiting function based on the screening method with threshold matrix, and investigate its potential application value. The algorithm and principle of minimum threshold matrix were researched and analyzed, the threshold matrix was improved after screening to generate microcosmic artistic effect. Design of threshold matrix with microcosmic artistic dot was realized at different angles, and the screening experiment was completed with computer. The screening images with microcosmic artistic morphology were obtained on different color plates. After the recombination of the four plates, the printing image got the ideal halftone effect. The microcosmic artistic dot designed based on minimum threshold matrix could realize the anti-counterfeiting purpose in the dot layer of AM, the system of artistic dot with micro-structure was established, and can be combined with other anti-counterfeiting technologies such as grating anti-counterfeiting.