The aim of this article was to analyze the causes for vibration of the cassette mechanism of the single-sided corrugating machine and the vibration reduction optimization was conducted. The dynamics model of the key cassette mechanism of the single-sided corrugating machine was established. The mesh center distance of the upper and lower corrugating roll was changing. Then the simulation of the modal analysis of the upper corrugating roll and lower corrugating roll was conducted in Ansys, and then a vibration test was conducted on the single-sided corrugating machine. Comparing the test results with the natural frequency obtained from the roller′ s modal analysis and the dynamic model established for the cassette mechanism, the primary reason of single-sided corrugating machine′ s vibration was found to be the resonance occurring at the frequency 154, 217 Hz, etc. Finally, the vibration reduction optimization of the cassette mechanism was conducted. All the above proved the effectiveness of studying the vibration characteristics of single-sided corrugating machine using the established dynamic model and simulation of Ansys.