This work was aimed to study the effect of sorbic acid on the properties of low density polyethylene (LDPE) film. Sorbic acid powder was added to low density polyethylene resin. By blending, extrusion, blow molding and other processes, antibacterial sorbic acid-LDPE film was prepared. And the effects of sorbic acid content on the tensile strength, optical properties, water vapor transmittance, microstructure and antimicrobial properties of LDPE film were studied. With the increase of sorbic acid content, the tensile strength of the film first increased and then decreased, the transparency decreased gradually and the haze gradually increased, the trend of permeability coefficient first decreased and then increased. More than 1.5% of sorbic acid content in the film had inhibitory effect on E. coli, while more than 2% of sorbic acid content had inhibitory effect on L. monocytogenes and S. aureus. In conclusion, the sorbic acid-LDPE Films have good packaging property and inhibitory effect, and can be used as a kind of good material for food packaging.