The aim of this work was to study the application of time-temperature Indicators in the transport packaging of vaccines. Two key factors in the failure of the vaccines in the process of storage and transportation, namely time and temperature, were introduced, as well as the research progresses in the time-temperature indicator both at home and abroad. And the application of TTI in vaccine transport packaging was explored. The vaccine is a special kind of commodity, which has strict requirements on environmental temperature in the process of transportation and storage. And the inefficacy of vaccine can′ t be directly identified by the sensory organs, with some concealment. After application of time-temperature Indicators in the transport packaging of vaccines, whether the vaccine is still within the validity period could be judged visually and accurately through the color change of the time-temperature indicators, vaccinating children against failure was avoided. Meanwhile, the waste of vaccines was also avoided. In other words, the time-temperature indicators could guarantee the quality of the vaccine, ensuring safety and effectiveness of vaccination.