A series of environment-friendly fluorescent gravure inks based on self-prepared phosphor were prepared by one-step process, and the properties of these inks were studied. Primary characterization of the phosphor was done, and the effect of the content of phosphor on the viscosity, fineness and adhesion of ink samples was explored, and the fluorescence properties of printed samples were also investigated in order to screen a formula whose properties were close to the commercial fluorescent gravure ink. The results showed that the phosphor was a kind of terbium complexes with nice performance of oil solubility, dispersibility and brightness. The printed samples were colorless under natural light, but showed bright green under ultraviolet light B. The viscosity of printed samples increased as the content of the phosphor increased, while the fineness and adhesion were not affected. All the sample inks showed high adhesion, strong heat resistance and light resistance. When the content of the phosphor was 2% , the fluorescence intensity of the strongest emission peak of printed samples was close to that of the commercial fluorescent gravure ink. In addition, the fineness, viscosity, adhesion degree, light resistance and heat resistance of the formula, without the introduction of benzene and ketone substances, matched the requirements of the national standards, thus it could be considered as the preferred formula.