This paper studied a combination of surfactants 6501 and 6502 which was added to fountain solution to replace alcohol so as to get an alcohol-free fountain solution. First, the original solution was prepared with fountain powder, then different concentrations of surfactants 6501 and 6502 were added to test the critical micelle concentration of the single system. According to the compounding principle and method, surfactants 6501 and 6502 were mixed and the best mix ratio and the optimum adding concentration were obtained, thus, a best formula fountain solution were gained. The best ratio was 4.36 : 5.54 for compound 6501 and 6502, and the critical micelle concentration was 0.1% at this ratio. Each performance parameter of this formula can meet requirements. Combination of surfactants 6501 and 6502 to replace alcohol is feasible to make alcohol-free fountain solution which solves the problem that alcohol causes hazards during dampening.