This study aimed to investigate the methods to obtain the vibration transmissibility curves of cushion materials. The principle, algorithm and concrete realization process of time and frequency area analysis methods were discussed. In the frequency area analysis process, the window function was used to reduce the energy leakage and the Welch method was applied to improve the variance characteristics of power spectrum estimate. The packaging system composed of mass and cushion material was inspired with the vibration table, vibration control device, A/D conversion board, acceleration sensors and other hardware in the sine sweep mode. The cushion material vibration transmissibility of two methods in time and frequency area analysis was obtained through analysis and treatment of the collected excitation signal and response signal. Based on the data processing and the principle of vibration transmissibility experiments, the cushion materials vibration transmissibility test system is developed with software LabVIEW. In addition, the established system was applied to draw the vibration transmissibility curves of two kinds of methods, and the two kinds of curves were similar. The test system is simple and has a friendly man-machine interface, which can provide a powerful tool for optimal cushioning and shockproof packaging design of cushion packaging material.