This experiment studied the effect of random defect on the load capacity and cushion properties of honeycomb paperboard. In this experiment, prefabricated honeycomb cardboards with different cell-wall removing ratios were prepared， and the quasi-static compression test was conducted to obtain the stress-strain curve, the relationship curve of cushioning coefficient and cell-wall removing ratios and the cover of initial peak stress and energy absorption. Based on the test results, the effect of defect rate on the load capacity and cushion properties of honeycomb paperboard was discussed. The results indicated that with the increase of cell-wall removing ratios, the material cushioning coefficient was increased in different degrees, defects would significantly decrease the initial peak stress, and the energy absorption properties tend to decline. This experiment provided a basis for optimized design of product packaging.