This paper studied preparation and properties of biodegradable and controllable bacteriostatic functional film. Loofah fiber extractive liquid (LF), microcrystalline cellulose (MCC), polyvinyl alcohol (PVA) and ethylene vinyl acetate copolymer (EVA) emulsion were blended with different ratios. The degradable and LF controllable bacteriostatic film was prepared by aqueous tape-casting technology, and the antibacterial and biodegradable properties of the material were analyzed. With increase of the concentration of LF in the casting film, the concentration of tannins and glycosides released from the film increased accordingly and the inhibitory performance of microorganisms was stronger. However, the basic properties of the film were affected when the concentration of LF was higher than 25%. When the amount of LF/MCC was 20% (mass fraction), the antimicrobial property of the casting film increased 61.5% compared to the controls, and 44.8% of components degraded in 20 days in compost. The relavent data test showed this packaging film was an ideal biodegradable material.