The natural plant essential oil, hygroscopic agent and modified atmosphere packaging technology were applied together in the preservation of Agaricus bisporus. At ambient temperature, 3 test groups were set as follows: in the combined application group, the PE bags were filled with O2(40%)+N2(60%), enclosed with 10 μL antibacterial agent (garlic essential oil : cinnamon oil = 1∶2) and 2 g hygroscopic agent (acrylic acid and acrylamide copolymer); in control group 1, Agaricus bisporus was preserved in the open air; in control group 2, Agaricus bisporus was preserved in the PE bags without any other packaging technology. Quality indexes of Agaricus bisporus such as sensory quality, weight loss ratio, browning degree, total phenol content and reducing sugar content were regularly tested. The application of combined packaging technology effectively improved the preservation quality of Agaricus bisporus by slowing down browning, reducing loss ratio, and inhibiting the decrease of total phenolics and reducing sugar content. The shelf life of Agaricus bisporus after 1 day exposure in the open air was extended to 8 days. In conclusion, the combined application of antibacterial agent, hygroscopic agent and modified atmosphere packaging technology was beneficial to improving the preservation quality of Agaricus bisporus, and significantly prolonged its shelf life.