The aim of this study was to accurately extract and resolve the dots of 3D micro-morphology in the process of printing transfer. On the basis of accurate collection of 2D dot data by the microscopic test system, the theory of Shape From Shading (SFS) was utilized to construct the 3D model of dot micro-morphology with different printing shape. The 3D form of the printing dot with different shape on the substrate surface was intuitively and accurately restored, and the difference in characteristics of 3D dot micro-morphology caused corresponding effects on the printing quality. The derivation process of SFS algorithm was precise, and the 3D model established had a relatively high accuracy, and could truly and meticulously reflect the specific form of dot transfer in the process of printing, which was therefore an effective method to restore the 3D dot form. Accurate acquisition and analysis of the 3D dot form could intuitively and effectively detect the dot transferring result as well as control the printing quality from the origin, which has become a new method to evaluate image replication quality.