To design a new kind of biosensor for detection of bisphenol A (BPA) based on the fact that the aggregation of gold nanoparticles (AuNPs) could be induced by Phthalic Diglycol Diacrylate (PDDA), and that oligonucleotides combine with PDDA to prevent AuNPs from aggregation, and the specificity of the anti-BPA aptamer. AuNPs solution containing anti-BPA aptamer and PDDA at a certain concentration was set as the reaction system. Upon the addition of BPA and reaction at room temperature, the specific wavelength of light absorption value changed along with the concentration of BPA which could be determined by UV-Vis spectrometry. Under the optimal conditions, a low limit of detection of 0.6 ng/mL was obtained in the range of 1～50 ng/mL. In addition, the specificity was outstanding among analogues of BPA. The recovery rate in lake water samples spiked with BPA could reach 95.6% to 108.0%. A convenient and sensitive method was successfully used to determine BPA, and the method has a certain application prospect for industrial production and food security in the trace detection of BPA.