Objective Reader collision is a common problem in RFID. The common anti-collision algorithm do not consider the two types of reader collision simultaneously and have high requirements of hardware, which influences the efficiency of data transmission in the process of RFID data communication. The aim of this paper was to propose an anti-collision algorithm based on schedule mode. Methods The algorithm was proposed based on the anti-collision of scheduling, which adopted the central server. The mixture integer programming model was established to optimize the resources among the readers. At 2 different reader distances, and considering both the throughput rate and the equality rate, the proposed algorithm was compared with the CSMA algorithm. Results When considering both the throughput rate and the equality rate, the algorithm based on schedule mode was superior to the CSMA algorithm. Conclusion This algorithm could enhance the throughput rate and the equality rate.