Objective To establish a method for the determination of quercetin retention in antioxidant food package film by HPLC. Methods Quercetin was extracted in ethanol at 65 ℃ for 10 h and the extraction was repeated once. Results The detection method was fast, and could effectively improve the peak shape as well as avoid peak tailing. The linear regression equation for quercetin was y=643.65x+0.0572 (R2=0.9995, n=6) and the linear range was 1.6~280 μ g/mL. The average recovery was 99.69% (RSD=1.77%) for quercetin. Conclusion This was a simple, rapid and accurate method to detect the quercetin retention in the antioxidant food package film.