Objective To prepare the water-soluble polyurethane hot melt adhesive. Methods Water-soluble polyurethane hot melt adhesive was prepared using polyethylene glycol (PEG) and toluene diisocyanate (TDI) as raw materials. The effects of PEG molecular weight, raw material ratio, TDI addition temperature, and reaction time on product properties were discussed. Results At a PEG8000∶PEG600∶PEG200∶glycol∶TDI molar ratio of 1∶1∶0.8∶1∶4, a TDI addition temperature of 60～ 70 ℃, and a reaction time of 4 h, the as-obtained product exhibited a softening point of 59.5 ℃, and an adhesive strength of 2.668 MPa. Conclusion This water-soluble polyurethane hot melt adhesive could be used as surface protection film of industrial semi-finished products.