Objective To study the migration of di-2-ethylhexyl phthalate(DEHP) in corn migrated from packaging film. Methods The determination of DEHP in corn migrated from packaging film was studied using GC/MS with ultrasonic assisted extraction method. Specifically, influencing factors upon sample extraction including various extraction solvents (hexane, methanol and dichloromethane), ultrasonic extraction time, ultrasonic extraction temperature and purification method were investigated. Results The results showed that samples could be extracted well with hexane for 30 min at 38 ℃ . Under the optimal conditions, the recovery rate of the method was between 88.53% and 103.28%, while the LOD was 2.54×10-3 mg/kg. Conclusion This method shortened the pretreatment time and improved the precision. It was therefore an effective way for the detection of DEHP in corn.