Objective To solve the problems that general barcode printers in the market could not label automatically, the printed label is labeled by hand, and the label is neither beautiful nor sanitary, an automatic tube labeling machine was designed and manufactured. Methods The tube labeling machine was modeled by CATIA software. The vice-roll, which is the weakest part of labeling machine, was analyzed in statics by Ansys, and the stress and the deformation of the vice-roll were investigated. Then the components were purchased and processed, and the labeling machine was assembled. At last, tubes with a diameter of 12 mm were labeled by the tube labeling machine. Results When the speeds of the main roll and vice-roll motor were 650 r / min and 195 r / min respectively, the linear velocity of the tubes can match with the paper-out speed of the barcode printer, and the labeling machine could label accurately. Conclusion The tube labeling machine is correct in theory, and it could label efficiently and accurately.