Objective To explore amylase time-temperature indicator( TTI) and its stability properties with the immobilized enzyme. Methods Based on the reaction of amylase, the optimal formula of the indicator at 4 ℃ and within 4 ~ 5 d was determined. After the employment of enzyme immobilization technology, the coagulation was observed, the enzyme activity and the absorbance value change with time curve when it was applied in TTI were analyzed. Results TTI formula was determined, and the activity decreased after the amylase was immobilized, but the absorbance value changed more smoothly. Conclusion The best formula applied to TTI at 4 ℃ was using 40 g / L malt batter and 0. 1% Iodine as the substrates, 3% agar immobilization enzyme solution as reactant, with the optimal pH value and temperature of 6. 0 and 55 ℃ , respectively. In this case, the highest stability and the optimal time and color changes were achieved.