Objective To investigate the vibration isolation characteristics of transport vehicle-nonlinear packaging system coupled under pulsed excitation of the road. Methods Comprehensively considering the nonlinear vibration transfer model on the road-vehicles-cushioning packing material-goods coupled system, a 5-DOF motion analysis theoretical model based on a half system was established for the vehicle-nonlinear packaging system under pulsed excitation of the road, and dynamic equation was derived and solved. According to the results, the effects of considering and not considering the coupling of the vehicle -nonlinear packaging system were compared. It was found that the maximum vertical displacement of the package under the coupled system of vehicle-nonlinear packaging system was about 1. 25 times as high as that of the system not considering the coupling, and the effects of nonlinear factors of isolation material and roughness of the road on the package were also analyzed. Results The nonlinear factors of isolation material and roughness of the road had some impact on the response of the package system during vehicle transport. Conclusion The results of this paper are very useful for the accurate design and effect analysis of vibration isolators.