Objective To design data transfer program for Medical Cold-Chain Monitoring System based on RFID. Methods First of all, the humiture information was collected and the conflict problem of Labels was solved; and then the transmission and communication problem during the data transmission from sensor to RFID reader and from GPRS module to central server was solved. Results Based on the UDP-protocol network transmission platform, acommunication interface program of CRC and anti-collision was designed and developed. Conclusion The real-timeness, integrity and accuracy of data collection and transmission were realized, which builds a solid foundation for the further development of Cold-chain Monitoring system.