Objective In order to combine the advantages of nano cellulose and graphene, in this paper, we used nanocellulose as matrix, and graphene oxide as reinforcing phase, fabricate the graphene oxide/nano cellulose composite film. Method The cellulose nanofibrils made by acid and alkali direct treatment method and acid and alkali alternate treatment. The graphene oxide made by one step oxidation method and cycle oxidation-expansion method. Then observed its morphology, found the best preparation process. Tested the tensile strength and wetting property of graphene oxide/ nano cellulose composite film, which made by the optimum process. Results The results showed that acid and alkali alternate treatment can obtain nano cellulose film which had clear structure, and the diameter of fibre was 50 nm. Cycle oxidation-expansion method can made graphene oxide with thin thickness at the nanoscale. When nanofiber and graphene oxide’s quality ratio was 20:1, the tensile strength of GO/nano cellulose conposite film could reach 149.68 MPa, compared with the pure cellulose film, whch increased 19.55%. and the contact angle of composite film is greater than the pure cellulose film. Conclusion It was confirmed that the graphene oxide can enhance nano cellulose film, to a certain extent, the barrier property of the composit film to water was superior to cellulose film.